亚洲a∨日韩av高清在线观看,亚洲国产一区在线观看,国产黄大片在线观看,一区二区三区四区中文字幕

您好!歡迎訪問上海起發(fā)實(shí)驗(yàn)試劑有限公司網(wǎng)站!
全國服務(wù)咨詢熱線:

15921799099

當(dāng)前位置:首頁 > 產(chǎn)品中心 > 自產(chǎn)產(chǎn)品 > 試劑 > 熱烈慶祝上海起福成為Phosphosolutions公司中國代理

熱烈慶祝上海起福成為Phosphosolutions公司中國代理

簡要描述:熱烈慶祝上海起福成為Phosphosolutions公司中國代理

  • 產(chǎn)品型號:
  • 廠商性質(zhì):代理商
  • 更新時(shí)間:2025-06-01
  • 訪  問  量:3550

詳細(xì)介紹

 公司概況

 

背景

基因工程-- Phosphosolutions是*代可以完整描繪人體的遺傳物質(zhì)序列的企業(yè)。

蛋白質(zhì)體學(xué)項(xiàng)目:Phosphosolutions是第二代試圖將所有體內(nèi)蛋白質(zhì)表達(dá)出來的企業(yè)。

PhosphoSolutions公司—第三步我們將超越蛋白質(zhì)體學(xué) 進(jìn)而 專注于磷蛋白質(zhì)。

 

our focus 專業(yè)特色

PhosphoSolutions公司專注于蛋白質(zhì)組學(xué)中的一個(gè)(10-20%)含量的小部分磷蛋白質(zhì)。磷蛋白是監(jiān)管控制組蛋白質(zhì)的關(guān)鍵,這一部分是被稱為phosphosome蛋白質(zhì)。磷蛋白被認(rèn)為是在神經(jīng)系統(tǒng)疾病如老年癡呆癥和癌癥方面的關(guān)鍵元素,實(shí)質(zhì)上,phosphosome是蛋白質(zhì)組學(xué)作物的精華。

 

公司目標(biāo)

簡明概述:我們要成為世界上的磷蛋白組的提供者。

方案#1, 特異性磷抗體:首先我們要準(zhǔn)備磷蛋白組。在激活或磷酸化狀態(tài)下磷蛋白組是蛋白質(zhì)識別研究中的*關(guān)鍵工具。

 

Antibodies 抗體

特異性磷抗體:Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations (Nestler and Greengard, 1984). Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies (Lerner, 1982;Sutcliffe et al., 1983), an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.

The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin (KLH) (Ross et al., 1981). Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase (Nairn et al., 1982). A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.

Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site(s) of phosphorylation (Czernik et al., 1991)). In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation (Czernik et al., 1991). Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art (Czernik et al., 1995;Czernik et al., 1996). Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.

參考文獻(xiàn)

1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P (1991) Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.

2. Czernik AJ, Mathers J, Mische SM (1997) Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.

3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM (1995) Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.

4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.

5. Nairn AC, Detre JA, Casnellie JE, Greengard P (1982) Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature (Lond ) 299: 734-736.

6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 

8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA (1983) Antibodies that react with predetermined sites on proteins. Science 219: 660-666.

主營產(chǎn)品清單如下:

Anti-14-3-3 Protein

 

Anti-14-3-3 Protein (Ser58)

 

Anti-ABCA4 (Rim Protein)

 

Anti-Actin

 

Anti-Adenylate Cyclase III-NEW!

 

Anti-Alpha II Spectrin-NEW!

 

Anti-Alpha Internexin (NF66)

Anti-alpha Synuclein

Anti-Alpha Synuclein (Ser129)

 

Anti-Amyloid Precursor Protein

Anti-Aquaporin2 (Ser264)

 

Anti-Aquaporin2 (Ser269)

 

Anti-ATF2 (Ser490,498)

 

Anti-ATF2 (Thr52)-NEW!

Anti-Beclin-1 (Ser234)-NEW!

 

Anti-Beclin-1 (Ser295)-NEW!

Anti-Calcitonin Gene Related Peptide (CGRP)

 

Anti-Calretinin

 

Anti-CaM Kinase II (Thr286)

 

Anti-CaM Kinase II (Thr305)

 

Anti-cdc2 (Tyr15)

 

Anti-CDK5

 

Anti-Choline Acetyltransferase

Anti-COBRA1 (NELF B)--NEW!

 

Anti-Collagen I, alpha 1, propeptide

 

Anti-Collagen I, alpha 1, opeptide

 

Anti-Connexin43

 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
上海起發(fā)實(shí)驗(yàn)試劑有限公司
地址:上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實(shí)驗(yàn)試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關(guān)注我們
歡迎您關(guān)注我們的微信公眾號了解更多信息:
歡迎您關(guān)注我們的微信公眾號
了解更多信息
亚洲a∨日韩av高清在线观看,亚洲国产一区在线观看,国产黄大片在线观看,一区二区三区四区中文字幕
久久伊人蜜桃av一区二区| 久久久久9999亚洲精品| 欧美videos大乳护士334| 中文字幕五月欧美| 日产欧产美韩系列久久99| 亚洲综合色丁香婷婷六月图片| 国产传媒一区在线| 国产一区二区三区免费看| 亚洲精品亚洲人成人网| 精品免费视频.| 国产呦精品一区二区三区网站| 一区二区三区在线播放| 中文子幕无线码一区tr| 久久精工是国产品牌吗| 麻豆视频观看网址久久| 亚洲一区二区视频| 国产原创一区二区| 中文字幕欧美区| 国产拍揄自揄精品视频麻豆| 亚洲一区二区三区爽爽爽爽爽| 国产夜色精品一区二区av| 五月天欧美精品| 久久久久久久久久久久久女国产乱| 亚洲国产精华液网站w| 99精品国产一区二区三区不卡| 7777精品伊人久久久大香线蕉超级流畅| 在线免费观看不卡av| 精品国产伦理网| 亚洲影视资源网| 国产成a人亚洲| 精品三级在线观看| 色天天综合久久久久综合片| 色综合网站在线| 亚洲人成电影网站色mp4| 亚洲视频在线一区| 欧美一区二区三区爱爱| 日韩一级黄色大片| 七七婷婷婷婷精品国产| 久久不见久久见免费视频1| 成人的网站免费观看| 精品国产第一区二区三区观看体验| 91亚洲精品乱码久久久久久蜜桃| 国产成人综合在线观看| 三级欧美韩日大片在线看| 亚洲成a人v欧美综合天堂| 亚洲欧洲av色图| 99国产精品99久久久久久| 国产欧美日韩激情| 91免费观看在线| 成人免费观看男女羞羞视频| 欧美日韩不卡视频| 国产精品第一页第二页第三页| 一本色道**综合亚洲精品蜜桃冫| 国产精品免费丝袜| 亚洲人妖av一区二区| 国产乱理伦片在线观看夜一区| 国产精品国产三级国产aⅴ入口| 国产精品美女www爽爽爽| 最新国产成人在线观看| 91在线porny国产在线看| 蜜臀久久99精品久久久画质超高清| 伊人一区二区三区| 成人听书哪个软件好| 欧美视频中文字幕| 久久国产成人午夜av影院| 欧美亚洲国产怡红院影院| 在线免费观看不卡av| 9i在线看片成人免费| 日本伊人午夜精品| 国产精品白丝在线| 亚洲视频1区2区| 久久毛片高清国产| 国产91富婆露脸刺激对白| 欧美人动与zoxxxx乱| 国产精品一区二区91| 成人av在线资源网| 精品亚洲aⅴ乱码一区二区三区| 一区二区三区四区高清精品免费观看| 欧美午夜在线观看| 欧美激情在线一区二区三区| 成人做爰69片免费看网站| 9191精品国产综合久久久久久| 亚洲一区在线视频观看| 91麻豆免费观看| 中文字幕在线观看一区二区| 不卡的电视剧免费网站有什么| 美日韩一级片在线观看| 久久久久国产精品麻豆ai换脸| 日韩视频在线一区二区| 久久久精品国产免费观看同学| 成人动漫在线一区| 国产精品色婷婷| 久久一区二区三区国产精品| 日韩精品色哟哟| 精品国产伦一区二区三区观看体验| 亚洲色图视频免费播放| 亚洲国产中文字幕| 91精品国产综合久久久久| 亚洲免费观看高清完整| 8x福利精品第一导航| 色爱区综合激月婷婷| 国产精品免费aⅴ片在线观看| 亚洲成人三级小说| 国产精品色一区二区三区| 久久91精品国产91久久小草| 99国内精品久久| 国产精品久久国产精麻豆99网站| 精品久久国产字幕高潮| 中文字幕高清不卡| aaa国产一区| 欧美日精品一区视频| 国产欧美日韩不卡| 欧美精选在线播放| 欧美二区在线观看| 成人精品亚洲人成在线| 欧美精品丝袜久久久中文字幕| 国产亚洲欧美一级| 精品欧美一区二区久久| 久久久综合精品| 成人黄色av网站在线| 欧美激情一区二区三区在线| 亚洲高清免费视频| 精品久久国产老人久久综合| 成人午夜视频免费看| 久久综合狠狠综合久久综合88| 国产三级久久久| 亚洲国产精品久久久久秋霞影院| 国产亲近乱来精品视频| 日本二三区不卡| 一区二区三区毛片| 视频一区免费在线观看| 欧美日韩成人综合在线一区二区| 2014亚洲片线观看视频免费| 国产一区亚洲一区| 国产精品福利电影一区二区三区四区| 国产一区二区在线电影| 91精品国产一区二区| 亚洲精品免费在线播放| 成人网男人的天堂| 国产91综合一区在线观看| 国产高清精品网站| 国产精品久久久一区麻豆最新章节| 亚洲图片欧美色图| 日韩精品福利网| 亚洲精品国产高清久久伦理二区| 国产91露脸合集magnet| 狠狠色狠狠色综合系列| 欧美亚洲综合在线| 国产久卡久卡久卡久卡视频精品| 欧美精品v国产精品v日韩精品| 一区二区三区免费观看| 制服视频三区第一页精品| 午夜欧美电影在线观看| 欧美日韩国产区一| 亚洲小说欧美激情另类| 亚洲精品美腿丝袜| 欧美日韩一区二区在线观看| 国产一区二区三区蝌蚪| av亚洲精华国产精华精华| 亚洲一区在线观看免费观看电影高清| www.在线欧美| 91亚洲精华国产精华精华液| 久久久久久毛片| 精品欧美久久久| 国产老肥熟一区二区三区| 精品欧美乱码久久久久久| 亚州成人在线电影| 国产老肥熟一区二区三区| 欧美日本在线播放| 日韩视频国产视频| 99久久综合狠狠综合久久| 国产欧美日韩一区二区三区在线观看| 亚洲精品成人在线| 精品国产成人在线影院| 国产福利精品一区| 国模一区二区三区白浆| 99久久免费视频.com| 欧美日本一区二区| 国产一区二区0| 日韩欧美一级特黄在线播放| 欧美电影免费观看高清完整版在| 亚洲成人综合在线| 不卡一区二区三区四区| 亚洲蜜桃精久久久久久久| 成人黄色免费短视频| 亚洲国产中文字幕在线视频综合| 国产精品美女久久久久aⅴ国产馆| 国产精品―色哟哟| 不卡一区二区在线| 日本久久电影网| 在线一区二区观看| 欧美一区二区三区日韩视频| 国产制服丝袜一区| 成人美女在线视频| 韩国成人精品a∨在线观看| 欧美精品第1页| 欧美一二三四在线| 国产一区日韩二区欧美三区| 亚洲精品一卡二卡| 久久综合丝袜日本网|